Thiobacillus ferrooxidans is the main bacteria used in biological desulfurization technology. What is the living condition of the strain? How can we improve the reaction efficiency of Thiobacillus ferrooxidans? Let's have a look.
Thiobacillus ferrooxidans is an important leaching microorganism, which is acidophilic and suitable for medium temperature environment. It widely exists in acidic mine water and acidic environment containing iron or sulfur. In recent years, it has been widely used in biological desulfurization technology. So what should we pay attention to in the cultivation of Thiobacillus ferrooxidans?
据研究表明：1.氧化亚铁硫杆菌适合在初始pH为2.0时生长, 此条件下, 细菌很快适应环境, 可以正常吸取营养物质, 合成新的原生质, 形成一定量细菌所必需的酶；
According to the research, 1. Thiobacillus ferrooxidans is suitable to grow at the initial pH of 2.0. Under this condition, the bacteria quickly adapt to the environment, can normally absorb nutrients, synthesize new protoplasts, and form a certain amount of enzymes necessary for bacteria.
2.氧化亚铁硫杆菌的佳培养温度是30℃.在这两个温度范围内, 酶活性高, 细胞的生物化学反应速度和生长速度较快; 但微生物机体的重要组成, 如核酸、蛋白质等对温度较敏感, 随着温度的升高可遭受不可逆的破坏.
2. The optimum culture temperature of Thiobacillus ferrooxidans is 30 C. Within these two temperature ranges, the enzyme activity is high, the biochemical reaction rate and growth rate of cells are fast; but the important components of microbial organisms, such as nucleic acid and protein, are sensitive to temperature and can be irreversibly destroyed with the increase of temperature.
3.当接种适量增大时, 培养基中的初始菌数增加, 氧化亚铁硫杆菌快速繁殖.但由于培养基中的营养物质有限, 加入过多的菌液影响细菌的生长繁殖.表明培养氧化亚铁硫杆菌的佳接种量为10%.
3. When the inoculation amount increased, the initial number of bacteria in the culture medium increased, and Thiobacillus ferrooxidans multiplied rapidly. However, due to the limited nutrients in the culture medium, excessive bacterial liquid was added to affect the growth and reproduction of bacteria. It showed that the optimal inoculation amount of Thiobacillus ferrooxidans was 10%.
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